Sensitive detection of botulinum neurotoxin types C and D with an immunoaffinity chromatographic column test.

نویسندگان

  • Frank Gessler
  • Katrin Hampe
  • Helge Böhnel
چکیده

A sensitive and specific immunoassay for the simultaneous detection of Clostridium botulinum type C (BoNT/C) and type D neurotoxin was developed. Goat anti-mouse immunoglobulin G was bound to polyethylene disks in a small disposable column used for this assay. The sample was preincubated together with monoclonal antibodies specific for the heavy chain of BoNT/C and D and affinity-purified, biotinylated polyclonal antibodies against these neurotoxins. This complex was captured on the assay disk. Streptavidin-poly-horseradish peroxidase was used as a conjugate, and a precipitating substrate allowed the direct semiquantitative readout of the assay, if necessary. For a more accurate quantitative detection, the substrate can be eluted and measured in a photometer. Depending on the preincubation time, a sensitivity of 1 mouse lethal dose ml(-1) was achieved in culture supernatants.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 71 12  شماره 

صفحات  -

تاریخ انتشار 2005